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. 1997 Aug 5;94(16):8551–8556. doi: 10.1073/pnas.94.16.8551

Figure 4.

Figure 4

One-dimensional spectral slices along the three frequency axes taken from two-dimensional spectra. The magnitudes of the principal elements of the relevant spin-interactions are shown with bold tick marks. (A) 15N chemical shift slice taken from the PISEMA spectrum in Fig. 2 at the 1H–15N dipolar coupling frequency of 8.0 kHz. The 15N amide chemical shift tensor elements, referenced to liquid ammonia at 0 ppm, are 64, 77, and 217 ppm for σ11, σ22, and σ33, respectively (40). (B) 1H–15N dipolar coupling slice taken from the PISEMA spectrum in Fig. 2 at a 15N chemical shift value of 205.0 ppm. The maximum 1H−15N dipolar coupling frequency at ν∥ is 9.7 kHz. (C) 1H chemical shift slice taken from a two-dimensional heteronuclear correlation spectrum at a 15N shift value of 205.0 ppm. The 1H amide chemical shift tensor elements, referenced to tetramethylsilane at 0 ppm, are 3, 8, and 17 ppm for σ11, σ22, and σ33, respectively (40).