Table 1.
Summary of the λmax values and shifts relative to parent pigment spectrum (in parentheses) in nanometers and in cm−1 (bottom lines) of the intermediates of the two counterion mutants, recombinant rhodopsin (COS rho) and rhodopsin
| Species | Rhodopsin* | COS rho† | E113D† | E113A/A117E† |
|---|---|---|---|---|
| Pigment | 498 20,080 | 500 20,000 | 509 19,646 | 490 20,408 |
| Batho | 529 (+31) 18,904 (1,176) | 531 (+31) 18,832 (1,168) | 540 (+31) 18,519 (1,127) | 525 (+35) 19,048 (1,360) |
| BSI | 477 (−21) 20,964 (884) | 479 (−21) 20,877 (877) | 495 (−14) 20,202 (556) | 453 (−37) 22,075 (1,667) |
| Lumi | 492 (−6) 20,325 (245) | 490 (−10) 20,408 (408) | 486 (−23) 20,576 (930) | 482 (−8) 20,747 (339) |
| Meta I | NA | NA | NA | 466 (meta I-like) 21,459 (1,051) |