Table 1.
Activity of P-TEN measured with tyrosine, serine, and threonine phosphorylated substrates
| Substrate | Activity, pmol/min per mg |
|---|---|
| Phosphotyrosyl substrates | |
| polyGlu4Tyr1 | 4,840 ± 140 |
| RCML | 88 ± 6.6 |
| EDNDYINASL peptide | 51 ± 4.0 |
| MBP | 21 ± 1.2 |
| Phospho-seryl and -threonyl substrates | |
| DSD peptide | 210 ± 4.2 |
| ETE peptide | 161 ± 1.8 |
| Casein (casein kinase II) | 14.2 ± 1.2 |
| Casein (protein kinase A) | 27.5 ± 2.2 |
| MBP | 11.3 ± 0.6 |
P-TEN activity was measured with the indicated substrates in triplicate. The activity is expressed as the mean pmol phosphate released per min per mg enzyme ± SD.