Figure 2.

pECA1 restored the growth of yeast pmr1 mutant on medium with Mn²⁺ but not of pmr2 mutant on Li-containing medium. Wild-type (W303), pmr1 (AA542), and pmr2 (K633) cells were transformed with either a high-copy plasmid vector (p426) alone or the vector containing the Arabidopsis ECA1 (pECA1). The cells were streaked on SC-URA plates containing glucose (Glu) or galactose (Gal) plus either 3 mM MnCl₂ (A) or 150 mM LiCl (B) and incubated for 4 days at 30°C.