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Nckβ is redistributed into neuronal processes after Reelin stimulation. (A and B) In cultures treated with control conditioned medium for 30 min, Nckβ (A; green) is found predominantly in the cell soma. (C and D) After Reelin treatment, Nckβ (C; green) is apparent in the majority of processes. Processes were detected (B and D) with fluorescently labeled phalloidin to visualize filamentous actin (red), and nuclei were detected with DAPI. Bar, 20 μm.
