FIG. 9.

Nck-enriched regions in distal processes of Reelin-treated neurons have patterns of actin filaments not observed in control-treated neurons. (A and B) The growth cones of neurons at 1 day in vitro demonstrate intense actin filaments (arrow) detected by phalloidin staining (A and B; red and white) and were devoid of Nckβ (green; A). (C to F) Approximately 5% of neurons had Nckβ enrichments in distal processes 10 min after Reelin stimulation (arrowheads). The Nckβ-enriched regions had patterns of actin filaments that varied from actin-poor (C and D) to intense peripheral actin features such as actin rings (star; E and F). Bar, 20 μm.