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. 2003 Oct;23(20):7122–7133. doi: 10.1128/MCB.23.20.7122-7133.2003

FIG. 5.

FIG. 5.

Caudal neural tube defects in PAK4-null embryos. (A and B) Cross sections of the caudal portions of neural tubes from wild-type (A) and PAK4-deficient (B) E10.5 embryos. In the PAK4-deficient embryo, what appears to be two neural tubes can be seen adjacent to a single notochord. A layer of neuroepithelium can be seen between the two lumens. (C to E) The expression of Pax7 (red) and Nkx6.1(green) was analyzed in cross sections of the caudal neural tubes of E9.5 wild-type (C) and PAK4-deficient (D and E) embryos. The dorsal-ventral patterning appears normal in the PAK4 mutant neural tubes (compare panel C with panels D and E). (F and G) Analysis of Shh (red) and Nkx6.1 (green) expression in cross sections of caudal neural tubes from E9.5 wild-type (F) and PAK4-deficient (G) embryos. Shh is expressed normally in a single notochord and floor plate of the mutant neural tube (see panel G). The results from panels C to G suggest the presence of a single improperly folded neural tube rather than two separate neural tubes.