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. 2003 Oct;23(20):7134–7142. doi: 10.1128/MCB.23.20.7134-7142.2003

FIG. 3.

FIG. 3.

Western blot analysis of PAK5 expression. (A) Western analysis of different tissue extracts from 8-week-old wild-type and knockout mice shows that the PAK5 protein is highly expressed in the brain. No PAK5 is found in the brains of the knockouts. The same blot was also probed with an actin antibody. (B) Western analysis of brain extracts from 8-week-old PAK5+/+, PAK5+/−, and PAK5−/− mice shows that PAK1, PAK2, and PAK4 protein are not abnormally upregulated in the absence of PAK5. The same blot was also probed with anti-actin antibody. (C) Northern analysis of total RNA from different tissues of 8-week-old wild-type and knockout mice. The blot was probed with a portion of PAK6 cDNA that is specific to PAK6. The same blot was also probed with actin as control. The results indicate that the PAK6 mRNA level is not changed in the absence of PAK5.