FIG. 7.

(A) CML268 cells transformed with pCM279 (adhp-IME1-3xHA), pCM388 (adhp-IME1-2xNLS-3xHA), or YCPlac22 (vector) were grown in YPA at 30°C. Samples were taken from exponential-phase cultures (exp). Then one part of the cultures was treated with 200 ng of rapamycin per ml, and samples were taken 30 min later (rap). Another part was transferred to sporulation medium and incubated for 3 h before the samples were collected (spm). RNA levels for the meiotic early genes IME2 and SPO13 were determined by Northern blotting. The TOR-regulated gene MEP2 was used as a control of rapamycin treatment. snU1 mRNA serves as a loading control. (B) Relative gene expression of samples in panel A. The loading was normalized to the U1 signal, and relative gene expression was determined by setting the maximal signal for each transcript to 100. (C) Schematic diagram of different elements involved in the regulation of the localization of Ime1. See Discussion for details.