FIG. 4.

Degradation of wt Cot and trunc-Cot by the 20S proteasome. Transcribed and translated wt Cot and trunc-Cot were incubated for the indicated times with 20S proteasome in the presence or absence of 10 μM lactacystin. The proteins were then resolved by SDS-PAGE and visualized by autoradiography, and the incorporated radioactivity was quantified by using an Instant Imager. The figure shows one representative experiment, and the graph shows the mean ± standard deviation of two experiments performed in duplicate.