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Rds3-1-TAP inactivation impairs Ist3p/Snu17p and Rse1p association with the Rds3p complex. Radiolabeled proteins were recovered by TAP purification (without gradient separation) from wild-type (Rds3-TAP) and mutant (Rds3-1-TAP) cultures grown constitutively at 30°C or shifted to 37°C for 1 h prior to harvest. The asterisk indicates the position of reproducible bands lost or greatly reduced with temperature shift.
