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Metabolic depletion of Yra1p does not impair pre-mRNA splicing. RNA was isolated from the GAL1::YRA1-dependent yeast culture grown constitutively in galactose (Gal) and after shift to glucose medium for the indicated periods of time. The RNA was fractionated on a denaturing formaldehyde gel and hybridized with probes for the intron-bearing RPS17A (formerly known as RP51A) transcripts, the intronless ADE3 mRNA, and the U2 snRNA.
