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Not all Yra1p is associated with Rds3p. A Western blot of extracts prepared from yeast that express Yra1-ProtA only or Yra1-ProtA and Rds3-TAP simultaneously was probed with antibodies to the common protein A component. Total, unfractionated extract; Bound, proteins released from calmodulin agarose. No protein A agarose was used as this would select for both tagged proteins. Approximately 10 extract equivalents were loaded in the Bound lane compared to the Total lane.
