FIG. 7.

Src and Fyn modify the association of plakoglobin to its cellular partners. RWP1 cells were cotransfected with 5 μg of pcDNA3.1His-plakoglobin and pCMV-Src (A) or pcDNA3.1His-Fer (B), with empty vectors as controls. After 48 h, cell extracts were prepared, His-tagged plakoglobin was purified by chromatography on nickel-agarose, and the associated proteins were analyzed with specific MAbs against α-catenin, E-cadherin, desmoplakin, desmoglein, and plakoglobin. WB, Western blotting; +, present; −, absent.