Figure 2.
Growth responses on YPD media supplemented with various end-product sterols (ergosterol or cholesterol), 3-ketosterols (ergosta-7,22-dien-3-one, cholesta-3-one, cholesta-7-en-3-one), and 4,4-dimethylsterols (4,4-dimethyl-cholesta-8,24-dien-3β-ol and lanosterol). Cells were pregrown on YPD+ergosterol medium anaerobically and serially transferred two to three times onto the indicated sterol- or sterone-supplemented media. Erg, ergosterol; lano, lanosterol; dmc, 4,4-dimethyl-cholesta-8,24-dien-3β-ol; 3-k-erg, ergosta-7,22-dien-3-one; chol, cholesterol; 3-k-chol, cholesta-3-one; 3-k-Δ7-chol, cholesta-7-en-3-one.