FIG. 4.

Nup96 colocalized with the spindle apparatus during mitosis, whereas Sec13 and Nup98 were distributed throughout the cell. Immunofluorescence and confocal microscopy were performed with HeLa cells at mitosis. Cells were fixed with formaldehyde, permeabilized with Triton X-100, and labeled with anti-Nup96 and anti-β-tubulin antibodies (A to C), with anti-Sec13 and anti-β-tubulin antibodies (D to F), or with anti-Nup98 and anti-β-tubulin antibodies (G to I). Merged images showed partial colocalization of Nup96 and β-tubulin at the spindle apparatus, whereas Sec13 and Nup98 did not colocalize with the spindles and was dispersed throughout the cell.