Skip to main content
. 2003 Oct;23(20):7271–7284. doi: 10.1128/MCB.23.20.7271-7284.2003

FIG. 7.

FIG. 7.

Interference with Sec13 dynamics by the Sec13-binding site of Nup96. (A) Plots show FRAP assays performed with HeLa cells cotransfected with Sec13-GFP and Nup96 (amino acids 201 to 427; the Sec13 binding site of Nup96), or Sec13-GFP and Nup96 (amino acids 428 to 849, which does not interact with Sec13). Measurements were performed as in Fig. 5 (n = 6). (B) Histograms show measurements by FRAP assays as in A of the total number of mobile Sec13-GFP molecules in the absence or presence of the Sec13-binding site of Nup96 at ratios of 1:1 or 1:5 or the carboxyl-terminal region of Nup96 (amino acids 428 to 849) at a ratio of 1:5.