FIG. 1.

Myostatin blocks BMP7-induced adipocyte differentiation. C3H 10T1/2 (A and B) and 3T3-L1 (C and D) cells were incubated for 10 days in the presence of various combinations of BMP2, BMP7, myostatin (10 nM), or no ligand (no TRT) as indicated. (A and C) Lipid accumulation was assessed in fixed cells by Oil Red O staining. (B and D) The effect of myostatin on the BMP2 (3 nM)- and BMP7 (3 nM)-regulated expression of key adipocyte transcription factors and late adipocyte markers was determined by reverse transcription-PCR analysis.