FIG. 1.

Prox1 interacts with various Ff1 isoforms in yeast and zebra fish embryos. (A) Interaction of Ff1 proteins with Prox1 in a yeast two-hybrid assay. β-Galactosidase (β-Gal) activities were calculated based on duplicate measurements of five independent yeast transformants. Note that Ff1b has an autonomous activation domain. (B) Ff1 proteins coimmunoprecipitate with Prox1. Capped mRNAs of FLAG-tagged Prox1 alone, or in combination with c-myc-tagged Ff1a, Ff1b, or Ff1c, were injected into zebra fish embryos at the one- or two-cell stage. The injected embryos were harvested at 16 hpf, and extracted proteins were biotinylated. The lysates were subjected to immunoprecipitation with anti-FLAG agarose beads. The immunoprecipitates were analyzed by Western blotting, and the specific protein bands were detected with streptavidin, FLAG-specific antibody, or c-myc-specific antibody.