FIG. 2.

Ff1 proteins interact with Prox1 through LXXLL motifs. (A) Schematic representation of Prox1 functional domains, including the three putative NR box motif sequences. The LXXLL and IXXLL motifs were mutagenized to (I/L)XXAA to generate seven full-length Prox1 NR box site-directed mutants. (B) The NR boxes of zebra fish Prox1 show differential interaction abilities with Ff1 proteins. Seven Prox1 NR box site-directed mutants were tested for their ability to interact with Ff1a, Ff1b, and Ff1c in yeast. The results are presented as mean and SEM (n = 5). NR1m, NR2m, and NR3m are single NR box mutants at the first, second, and third NR box, respectively. NR1m2m, NR1m3m, NR2m3m, are double NR box mutants, while NR1m2m3m is the triple NR box mutant.