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The heptad 9 and AF2 domains of Ff1 proteins are required for interaction with Prox1. (A) Mutations of Ff1 proteins at hepted 9 and AF2 were generated by site-directed mutagenesis. hep9A and hep9B are heptad 9 site-directed mutants with either LL or LP replaced by AA. (B) Interaction of Prox1 with the hep9A, hep9B, and AF2 mutants of the three Ff1 proteins in yeast. The results are from one representative experiment (n = 3) and presented as mean and SEM (n = 5).
