FIG. 8.

Effects of ff1bMO and prox1MO on the enzymatic activity of 3β-Hsd. Either ff1bMO or prox1MO was injected into one-cell-stage embryos (1 pmol per embryo). Uninjected and injected embryos were fixed at 31 hpf and subjected to chromogenic staining of 3β-Hsd activity (blue) and in situ hybridization for either proinsulin (red) (A to F) or ff1b (red) (G to L). The cluster of proinsulin-expressing cells is situated immediately caudal to the interrenal primordium, which expresses both ff1b and 3β-Hsd. Bar, 50 μm.