TABLE 1.
Number of zebra fish larvae retaining 3β-Hsd activity in the interrenal tissues after injection with various antisense MOs
| MO injected | Dosage (pmol) per embryo | No (%) with 3β-Hsd activity in interrenal tissue:
|
n | ||
|---|---|---|---|---|---|
| Complete loss | Partial loss | Wild type | |||
| ff1b | 1.0 | 212 (88.0) | 10 (4.1) | 19 (7.9) | 241 |
| 1.2 | 170 (98.2) | 1 (0.6) | 2 (1.2) | 173 | |
| prox1 | 1.0 | 2 (1.3) | 116 (73.4) | 40 (25.3) | 158 |
| 1.2 | 3 (3.2) | 88 (93.6) | 3 (3.2) | 94 | |
| ff1b + prox1 | 1.0 each | 104 (100) | 0 (0) | 0 (0) | 104 |
| STDa | 1.2 | 0 (0) | 1 (0.6) | 160 (99.4) | 161 |
| Uninjected | 1 (0.5) | 7 (3.7) | 181 (95.8) | 189 | |
a
STD, standard control MO.