TABLE 2.
Number of ff1b-MO-injected zebra fish larvae retaining prox1 expression in the interrenal tissue
| MO injected | Dosage (pmol) per embryo | No. (%) with prox1 expressionc:
|
n | |||
|---|---|---|---|---|---|---|
| Complete loss
|
Wild type
|
|||||
| ff1b(+) | ff1b(−) | ff1b(+) | ff1b(−) | |||
| ff1b | 0.8 | 44 (67.7) | 0 (0) | 21 (32.3) | 0 (0) | 65 |
| 1.2 | 86 (48.9) | 65 (36.9) | 25 (14.2) | 0 (0) | 176 | |
| 2.5 | 69 (38.1) | 92 (50.8) | 20 (11.1) | 0 (0) | 181 | |
| iaff1aa | 1.2 | 0 (0) | 0 (0) | 53 (100) | 0 (0) | 53 |
| STDb | 1.2 | 0 (0) | 0 (0) | 82 (100) | 0 (0) | 82 |
| Uninjected | 0 (0) | 0 (0) | 189 (100) | 0 (0) | 189 | |
a
iaff1a, the complement of the antisense sequence of ff1b-MO.
b
STD, standard control MO.
c
ff1b(+) denotes no or minimal reduction of ff1b transcripts at interrenal tissue; ff1b(−) denotes significant or total reduction of ff1b transcripts at interrenal tissue.