och1Δ mutants have cell division and glycosylation defects. (A) Morphology of och1 cells. Wild-type (PY1181) panels are i, iii, v, and vii. och1–1 (BLY77) panels are ii, iv, vi, and viii. (i and ii) Nomarski optics. (v and vi) Parallel DAPI staining. (iii and iv) Tubulin detected with YOL1/34. (vii and viii) Parallel DAPI staining. (B) Cell-cycle tally of wild-type and och1 cells. Strains are wild type (PY1181), och1–1 (BLY77), and och1–2 (BLY118). Similar results were found for och1Δ. (C) Immunoblot of invertase from wild type (PY1181), ste11Δ (BLY21), och1Δ (BLY315), and och1–1 (BLY77).