Table 1.
Titers of pseudotyped vectors determined by X-Gal staining
env gene | Target cell | Titer, cfu/ml |
---|---|---|
Ampho MuLV | 293 | 6 × 105 |
HeLa | 5 × 105 | |
HeL–CD4+ | 4 × 105 | |
C8166 | n.d. | |
HIV-1+ | 293 | <1 |
HeLa | <1 | |
HeLa–CD4+ | <1 | |
C8166 | n.d. | |
HIV-1 truncated‡ | 293* | <1 |
HeLa | <1 | |
HeLa–CD4+ | 9 × 104 | |
C8166* | 3 × 104 | |
BJAB | <1 | |
BJAB–CD4i | 3 × 103 | |
BJAB–CD4i | 1.7 × 104 | |
Jurkat | 2 × 104 |
X-Gal, 5-bromo-4-chloro-3-indolyl β-d-galactosidase. n.d., not determined.
Pool of hygromycin resistant colonies.
‡ Infections carried out using supernatants from cell clone TELCeB6/pTr712-9. Cells marked with * were infected using supernatants from clone TELCeB6/pTr712-K14.