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. Author manuscript; available in PMC: 2008 Apr 15.
Published in final edited form as: J Immunother. 2003;26(4):332–342. doi: 10.1097/00002371-200307000-00005

TABLE 2.

Multiple patterns of tumor cell line recognition were displayed by TIL cultures generated independently from fragments of tumor 2035

Melanoma cell lines*
888
A2− 		938
A2− 		526
A2+ 		624
A2+ 		1938
Autologous		 Ag†
TIL 2035
 F6 80 64 415 559 618 MART-1
 F8 99 378 24 192 22 None detected
 F15 13 1 19 14 770 Unknown
 F16 396 125 377 499 457 None detected
 F17 24 2 148 18 424 Unknown
 DF3 48 7 695 584 1939 MART-1
 DF4 4 5 12 42 1668 Unknown
 DF6 1 0 11 2214 1473 NY-ESO-1
 DF8 21 4 20 2810 1947 NY-ESO-1
 DF13 27 6 739 704 1481 MART-1
 DF17 203 32 57 86 1785 Unknown
*

Values indicate IFN-γ release (pg/mL) by TIL when stimulated overnight with the indicated melanoma cell line. Specific recognition of 526mel, 624mel, or 1938mel was defined as IFn-γ release greater than 100 pg/mL and at least twice the greater value of the HLA-A2− cell lines, and is indicated by values that are italic.

†

Antigen reactivity was determined subsequently. The HLA-A2 restricted peptide epitopes from MART-1 and NY-ESO-1 have been described previously,10 and additional unknown antigen recognition by some TIL is not excluded.18 The molecular identity of the unknown antigen is currently being investigated.