TABLE 3.
Multiple patterns of tumor cell line recognition were displayed by independent TIL cultures generated from fragments of 5 tumor specimens resected from a single patient; the variation in activity among independent TIL derived from a single lesion is as great as the variation in activity among TIL derived from different lesions
| Tumor cell line stimulator* |
||||||
|---|---|---|---|---|---|---|
| HLA-A2− |
HLA-A2+ |
|||||
| Biopsy | TIL line | None | 888 | 938 | 526 | 624 |
| 1 | 1926-1 F7 | 9 | 32 | 6 | 120 | 357 |
| 1926-1 F8 | 10 | 270 | 54 | 359 | 662 | |
| 1926-1 F9 | 3 | 194 | 50 | 68 | 149 | |
| 1926-1 F10 | 0 | 126 | 48 | 74 | 170 | |
| 2 | 1926-2 F2 | 1 | 0 | 0 | 19 | 19 |
| 1926-2 F4 | 6 | 231 | 184 | 832 | 2042 | |
| 1926-2 F5 | 16 | 97 | 65 | 92 | 137 | |
| 1926-2 F6 | 1 | 43 | 14 | 753 | 862 | |
| 1926-2 F10 | 5 | 1 | 0 | 28 | 40 | |
| 3 | 1931-1 F1 | 138 | 181 | 92 | 76 | 113 |
| 1931-1 F6 | 61 | 52 | 20 | 13 | 19 | |
| 4 | 1931-2 F2 | 28 | 1378 | 200 | 870 | 1440 |
| 1931-2 F3 | 43 | 319 | 48 | 175 | 301 | |
| 1931-2 F4 | 14 | 114 | 38 | 1473 | 2327 | |
| 1931-2 F5 | 72 | 198 | 53 | 2016 | 2744 | |
| 1931-2 F6 | 11 | 39 | 13 | 57 | 126 | |
| 1931-2 F11 | 21 | 821 | 211 | 117 | 409 | |
| 5 | 1942 F1 | 29 | 57 | 12 | 3183 | 3132 |
| 1942 F2 | 56 | 29 | 22 | 284 | 399 | |
| 1942 F3 | 28 | 25 | 23 | 20 | 58 | |
| 1942 F4 | 11 | 3 | 3 | 418 | 364 | |
| 1942 F5 | 128 | 64 | 20 | 90 | 138 | |
| 1942 F6 | 38 | 232 | 42 | 55 | 99 | |
*
Values indicate IFN-γ release (pg/mL) by TIL when stimulated overnight with the indicated melanoma cell line. Specific recognition determined as in Table 2.