TABLE 4.

Quantitatively different patterns of peptide antigen specificity and tumor cell recognition were displayed by TIL cultures generated from a single-cell suspension of tumor 2009

		T2/peptide*		Melanoma line†		Tumor digest‡
	Media	MART	g209	888	624	1866	2009
Controls§
Media	0	68	0	3	2	0	0
CK3H6	3	5	2785	17	2210	179	878
JB2F4	3	18,500	5	6	6290	75	420
TIL 2009
W1	4	4470	34	250	7190	220	4730
W2	2	8790	25	35	3910	27	811
W3	0	7390	26	14	2180	33	1860
W4	0	116	19	71	904	63	829
W5	6	14,200	77	14	7610	52	1300
W6	5	405	60	11	>10,910	182	4330

^*Values indicate IFN-γ release (pg/mL) by TIL when stimulated with the indicated melanoma cell line. Specific recognition determined as in Table 2. The HLA-A2 binding peptide epitopes MART (MART-1:27-35) and g209 (gp100:209-217) were pulsed at 1.0 μmol/L concentration onto T2 cells, then used as stimulators.

^†Melanoma cell line 888 was HLA-A2⁻ and line 526 was HLA-A2⁺.

^‡Tumor digests were cryopreserved immediately after preparation, and thawed for use as stimulators on the day of the coculture assay. Number 1866 was from an HLA-A2⁻ patient and number 2009 was from the autologous patient. Strong recognition of tumor number 2009 by some TIL was later determined to be mediated by TRP-2 reactive T cells.

^§CK3H6 and JB2F4 are previously characterized cloned T cells with HLA-A2-restricted specificity for the peptide epitopes shown.