TABLE 5.

TIL cultures derived from a single patient demonstrate substantial variation in their CD4+ and CD8+ cell compositions

	Cytokine release*				FACS analysis†
TIL culture	888	938	526	1824	CD4+	CD8+
1921 F4	17	4	1168	1516	70.0	37.2
1921 F5	14	30	711	749	36.8	54.6
1921 F6	22	15	382	569	46.0	54.8
1921 F11	271	236	1188	1099	19.2	42.6
1941-1 F4	0	0	2590	2529	0.3	97.6
1941-1 F6	21	5	2334	2363	2.1	96.3
1941-3 F4	72	41	889	1283	46.0	53.7

^*Values indicate IFNγ release (pg/mL) by TIL when stimulated with the indicated melanoma cell line. Specific recognition determined as in Table 2.

^†Value indicates the percentage of viable lymphocytes in each TIL culture expressing the indicated phenotype. Values may be different from 100% due to double positive or double negative cells in the TIL culture.