Figure 2.

OT-I cells are deleted in RIP-OVA^hi but not RIP-OVA^lo mice. A total of 5 × 10⁶ Rag-1-deficient OT-I cells were adoptively transferred into RIP-OVA^hi mice, RIP-OVA^lo mice, or nontransgenic B6 mice. Four weeks later, the total number of OT-I cells remaining in the spleen and the LNs of the recipient mice was determined by flow cytometry, as previously described (4). The bars indicate the average number of OT-I cells found in the respective groups. These results are representative of three such experiments.