Figure 3.

OT-I cells are not anergized in RIP-OVA^lo mice. A total of 5 × 10⁶ Rag-1-deficient OT-I cells were adoptively transferred into RIP-OVA^hi mice (□, two recipients shown), RIP-OVA^lo mice (●, two recipients shown), and a nontransgenic B6 mouse (▵). After 4 wk, various numbers of LN cells were restimulated with 1500-cGy-irradiated spleen cells pulsed with the OVA_257–264 peptide at a concentration of 5 μg/ml. After another 3 days, their proliferative response to OVA was determined by [³H]thymidine incorporation. These results are representative of two such experiments.