Figure 1.

Characterization of RAG2^−/− ES cells by means of R2DBC. (A) Somatic chimeras were generated by injection of R2^BL/5.1 ES cells into RAG2^−/− blastocysts; peripheral blood (PB) isolated from chimeras and RAG2^−/− controls was analyzed by FACS after staining with FITC-anti-Ly5.1 or FITC-anti-Ly5.2. Ly5.1^low cells (RAG2^−/− panel) and Ly5.2^low cells (RAG2^−/− + R2^BL/5.1 ES panel) represent a population of leukocytes reproducibly identified in RAG2^−/− mice, which stain nonspecifically with both FITC-anti-Ly5.1 and anti-Ly5.2. (B) R2^BL/5.1 ES cells transfected with the pLCK-Ras^V12 construct were assayed by R2DBC. FACS analysis was performed on thymocytes (TH) isolated from chimeras and RAG2^−/− controls after staining with PE-anti-CD4 and CyC-anti-CD8. Data shown are from representative chimeras.