Figure 2.

Differential rescue of B and T cell development in RAG2^−/− chimeric mice with RAG2 genomic fragments. (A) Schematic of the λ13 genomic clone containing the RAG2 gene in relation to the genomic RAG locus. Restriction sites used for the generation of truncations are shown (N, NotI; C, ClaI; H, HindIII; S, StuI; R, EcoRI). Indicated below are the complete genomic fragment (13NN) and various truncations (13SN, 13SC, 13HC, and 13RS) tested in this study (see text for details). A summary table is shown to the right of the constructs to indicate the number of independent R2^BL/5.1 ES clones that rescued B and T cell development for each construct; “*” indicates the presence of a small number of DP/SP thymocytes or peripheral T cells in these chimeras, but minimal overall T cell rescue. (B) R2^BL/5.1 ES cells transfected with the 13NN, 13SN, 13SC, 13HC, or 13RS genomic fragments were assayed by R2DBC. Bone marrow (BM), thymus (TH), and spleen (SP) cells isolated from the various chimeras and RAG2^−/− controls were stained with (i) FITC-anti-Ly5.1, (ii) PE-anti-IgM and CyC-anti-B220, or (iii) PE-anti-CD4 and CyC-anti-CD8 and analyzed by FACS. Data shown are from representative chimeric mice.