Figure 2.

Characterization of xenomitochondrial cybrids. Total DNA purified from xenomitochondrial cybrids and parental cell lines was digested with PvuII and analyzed by Southern blotting using a human mtDNA-specific probe (Upper) and used as templates for a PCR using primers flanking the myotonic dystrophy polymorphic repeat (Lower). The radioactive PCR products were separated by 6% PAGE and exposed to an x-ray film. The figure shows that the xenomitochondrial cybrids having common chimpanzee (HC), pigmy chimpanzee (HP), and gorilla (HG) mtDNAs have a human nuclear background. 143B is a human osteosarcoma cell line. Positions of molecular weight markers are shown on the right.