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. 1999 Oct 26;96(22):12737–12742. doi: 10.1073/pnas.96.22.12737

Figure 1.

Figure 1

A dominant negative form of SREBP-1c decreases glucokinase gene expression in cultured hepatocytes of adult rats. Hepatocytes were cultured for 16 h after plating in the presence of 5 mM glucose. Cells were then incubated with 100 nM insulin and 5 mM glucose either without adenovirus or with null adenovirus (Ad null; 30 pfu per cell) or the SREBP-1c-DN adenovirus (3 or 30 pfu per cell). After 18 h, total RNAs were extracted and probed for the expression of glucokinase (GK), SREBP-1c DN and AGE genes, and 18S rRNA (18S). The Northern blot presented is representative of three experiments.