Figure 3.

Cellular distribution of HsCen3p. Cells were triple stained with rabbit anti-Cdc31p Abs (A), an anti-PCM mAb (CTR 453, B), and 4′,6-diamidino-2-phenylindole (DAPI) (C), or with rabbit anti-Cdc31p Abs (D), an anti-α-tubulin mAb (E), and DAPI (F). A 6-fold magnification of the duplicated centrosome framed in A and B is shown on the bottom right of A and B. Note that the two distinct pairs of closely associated dots stained with anti-Cdc31p Abs correspond to one pair of duplicated centrioles. Note that the midbody joining the two sister-cells shown in D–F is slightly stained with the anti-Cdc31p Ab. (Bars = 10 μm.)