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. 1997 Aug 5;94(16):8691–8696. doi: 10.1073/pnas.94.16.8691

Figure 1.

Figure 1

(A) SDS/PAGE gradient (5–20%) gel of precipitates formed 1.5 h after mixing 0.1, 1.0, 2.5, 5, 10, 25, or 50 μl of SEP with different 25-μl aliquots of a common plasma pool. Shown beneath the gel is a graph of protein content (micrograms) of precipitates from each lane. The results are explained in the text. Note the presence of a 49-kDa band derived from SEP in lanes receiving higher doses of SEP. Mr markers (kDa) are indicated. (B) SDS/PAGE gradient (5–20%) gel showing that, after exposure to SEP-free medium, both ppt 1 and ppt 2 remained in an insoluble form in a pellet (lane 1); they were not solubilized, and thus were absent from the supernatant (lane 2). In contrast, after exposure to SEP, components of ppt 1 and ppt 2 were solubilized and thus were not retained in the pellet (lane 3) but appeared in the supernatant (lane 4). Polypeptides smaller than 65 kDa are from SEP. Mr markers (kDa) are indicated.