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. 1997 Aug 5;94(16):8697–8701. doi: 10.1073/pnas.94.16.8697

Figure 1.

Figure 1

Generation of anti-HEL, anti-Id, and anti-anti-Id Ab. Arrows indicate immunizations. Immunization with HEL generated mAb D1.3 (Ab1); immunization with the Ab1 D1.3 generated the mAb E5.2 (Ab2, anti-Id), and, in rabbits, the polyclonal Ab2, anti-Id; immunization with the rabbit anti-Id generated the anti-anti-Id mAbs AF14 and AF52 (Ab3). mAb (D1.3, E5.2, AF14, and AF52) were raised in BALB/c mice. Anti-Id sera were raised in New Zealand Black rabbits (see Materials and Methods). AF14 and AF52 mimic D1.3 in their capacity to react with the antigen HEL and with the anti-Id E5.2. mAb E5.2 (anti-Id, Ab2) mimics the antigen HEL as described (10, 11).