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Equilibrium association constants of the reactions among D1.3, AF14, and AF52 with the anti-Id E5.2 and the antigen HEL
| mAb, Fab | Ka, FabE5.2, M−1 | Ka, HEL, M−1 |
|---|---|---|
| D1.3 | 1.4 × 109 | 1.0 × 108 |
| AF14 | 1.0 × 109 | 1.8 × 106 (1.6 × 106) |
| AF52 | 2.4 × 107 | 3.8 × 106 |
All values were obtained by plasmon resonance techniques except the one in parentheses, which was obtained by ultracentrifugal sedimentation equilibrium analysis. See Materials and Methods.
