Figure 5.

Boosting with MV-HA DNA or ALVAC-HA in mice primed at 1 week of age with ALVAC-HA induces similarly high levels of IL-5 and low levels of IFN-γ secretion by vaccine T cells. Splenocytes were harvested 3 weeks after boosting with ALVAC-HA or MV-HA DNA. Cytokine content was measured by capture ELISA after in vitro restimulation with the corresponding vaccine antigen and expressed by reference to standards. Antigen-specific cytokine secretion was obtained by subtracting the cytokine content present in the supernatant of splenocytes incubated with DMEM alone.