Figure 4.
Complete cDNA and amino acid sequence of human CBP. The arrowheads indicate the two different fusion junctions in the MLL-CBP chimeric cDNA. The three cysteine/histidine-rich regions are shown by circling the C and H residues. The second cysteine/histidine-rich region forms a C4HC3 finger, hence only the zinc coordinating residues are circled here. This C4HC3 finger is part of the minimal area, which contains the histone acetyltransferase (HAT) activity. This HAT-containing region is indicated by shading. The first boxed area (amino acids 1,106–1,170) corresponds to the bromodomain, and the second boxed area (amino acids 1,852–2,411) to the Q-rich carboxyl terminus. The PCR primers used for RT-PCR analysis are indicated by arrows under the appropriate sequence.