Figure 3.

(A) Immunoblot (autoradiogram) analysis of Gα_q subunit in platelet membranes from patient (P) and two normal subjects (N1 and N2). Platelet membrane proteins (22.5 and 45 μg) resolved by SDS/10% PAGE were transferred to Immobilon-P transfer membrane, and the blot was incubated with Gα_q antisera. The blot was probed with ¹²⁵I-protein A to visualize the Gα_q protein band on autoradiography. (B) Immunoblot (autoradiogram) analysis of Gα_i2 subunit in platelet membranes from patient (P) and five normal subjects (N1–N5). Platelet membrane proteins (22.5 μg) resolved on SDS/10% PAGE were transferred to Immobilon-P transfer membrane, and the blot was incubated with Gα_i2 antiserum (no. 1521). The blot was washed and probed with ¹²⁵I-protein A to visualize the Gα_i2 protein band on autoradiography.