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. 1997 Aug 5;94(16):8750–8755. doi: 10.1073/pnas.94.16.8750

Figure 4.

Figure 4

Flow cytometry analysis of GPIIb-IIIa complex on platelets from normal donors (N) and patient (P) in the resting (R) state and after activation. (Top Left) Binding of FITC-labeled mAb 10E5 (which recognizes both activated and resting forms of GPIIb-IIIa). (Top Right and Middle) Binding of PAC1 (which binds only to activated form of GPIIb-IIIa) after activation with TRAP, ADP, or PAF. (Bottom) Binding of Ab62 (anti-LIBS) after activation with ADP and PAF. Samples were analyzed on an Epics flow cytometer. Antibody binding to platelets was measured as fluorescence intensity, shown on the abscissa, and platelet count is on the ordinate. The data are representative of one to four experiments performed on separate occasions.