Table 3.
Q-PCR confirmation of microarray data.
| Micro-array | Q-PCR | |||||
| Gene name | Gene symbol | Sequence ID | Ratio* | Ratio* | SEM ± | p-value† |
| Mitochondria | ||||||
| Complex I | ||||||
| NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 | Nduf9b | XM_216929 | 1.47 | 1.29 | 0.05 | 0.001 |
| Complex II | ||||||
| Succinate dehydrogenase complex, subunit B, iron sulfur (Ip) | Sdhb | XM_216558 | 1.43 | 1.32 | 0.05 | <0.001 |
| Complex III | ||||||
| Ubiquinol-cytochrome c reductase binding protein | Uqcrb | XM_001074024 | 1.53 | 1.28 | 0.05 | 0.005 |
| Complex IV | ||||||
| Cytochrome c oxidase subunit VIIb | Cox7b | NM_182819 | 1.61 | 1.36 | 0.06 | 0.001 |
| Complex V | ||||||
| ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G | ATP5g | XM_001075306 | 1.52 | 1.15 | 0.05 | 0.11 |
| Metabolism and TCA cycle | ||||||
| Aldo-keto reductase family 1, member B8 | Akr1b8 | NM_173136 | 2.13 | 2.15 | 0.22 | 0.006 |
| Malic enzyme 1 | Me1 | 1.65 | 1.76 | 0.11 | <0.001 | |
| Mitochondrial ribosomes | ||||||
| Mitochondrial ribosomal protein S16 | Mrps16 | XM_001064095 | 1.43 | 1.20 | 0.05 | 0.03 |
| Mitochondrial protein transport | ||||||
| Translocase of inner mitochondrial membrane 8 homolog b | Timm8b | NM_022541 | 1.45 | 1.19 | 0.06 | 0.09 |
| Protein turnover | ||||||
| Proteasome subunit alpha type 3-like | Psma31 | BN000326 | 1.39 | 1.24 | 0.04 | 0.002 |
| Mucosal barrier | ||||||
| Phospholipase A2, group IIA (platelets, synovial fluid) | Pla2g2a | NM_031598 | 3.73 | 4.70 | 0.87 | 0.03 |
| Trefoil factor 3 | Tff3 | NM_013042 | 1.7 | 1.21 | 0.05 | 0.04 |
| Top 10 gene | ||||||
| Retinol binding protein 7 | Rbp7 | XM_575960 | 2.06 | 3.72 | 0.36 | <0.001 |
| Peptide hormone | ||||||
| Proglucagon | Gcg | NM_012707 | 2.56 | 2.91 | 0.23 | <0.001 |
* Ratio FOS diet/Control diet.
± SEM of Q-PCR data is given (n = 12 per group)
† p-value of Q-PCR data is given, the p-value of microarray data were all <0.001.