Table 6.
Primer sequences used for RT-PCR
| Representative clone | Primer sequence | Product size (bp) | |
| Highly expressed in BPH | |||
| MB3851 | forward | GGCGTGTTGGTGTTGCTTCA | 515 |
| reverse | GCTTAGCAAATCCTGGGACA | ||
| Female gonad-specifically expressed | |||
| AB0551 | forward | AATCATCGCTGCTTTCCTCGCC | 437 |
| reverse | GGGAGCAAGGAGAGGCTATTAC | ||
| OB0570 | forward | GGGACACAGACTTAACTTGGCG | 505 |
| reverse | AGCAACTCTTGCTCCATAGT | ||
| OB0998 | forward | TCCCAAAGTCGATGGCCGAATC | 529 |
| reverse | TGGTACTGACTGATCGTTCC | ||
| OB1013 | forward | GACGGAGCAACCGTACATAT | 486 |
| reverse | CGGTGAAGTAGATGGTCTCA | ||
| OB1061 | forward | CATCAGCCCTGTGACGATAGAG | 602 |
| reverse | TCTGCACTCGCTGCTGTTCCAT | ||
| OC0606 | forward | CTGTGATCTCAAGCTCAGCCTG | 498 |
| reverse | CACCAGCATGACCTTGGAGTAC | ||
| OC0638 | forward | GTCTGTGCCCTTTGCGCCAT | 504 |
| reverse | GCATGGCTGATGGCAGACTG | ||
| OC0681 | forward | GTAAAATGGCCGCCAAGTCACT | 435 |
| reverse | GAATGGAGAGGTGTGTTGCA | ||
| OC1014 | forward | CGTTGTTGCTGCCTTGGCACTA | 480 |
| reverse | CCTGGATTTGCCTAAGCTGCAA | ||
| OC1083 | forward | CATCCTGTGGATGCGGATACTC | 512 |
| reverse | GACTAGTAAAGCACAGATCG | ||
| OC2756 | forward | AGTGATCACAAGCTCTGCCTG | 382 |
| reverse | TATGCCACACAGCCAACACCAG | ||
| Male gonad-specifically expressed | |||
| AC0657 | forward | CGAGCTCTGTGTTGCTGAAG | 575 |
| reverse | CAGCAGGAGTTAAAACATGGG | ||
| AC0743 | forward | CACCAACTCAACACTCCAC | 525 |
| reverse | TTACTTCGGCCACACGGCTC | ||
| AC0825 | forward | GCGACTGGTGCAAAGTAGCA | 610 |
| reverse | TCTTGACTCAGCTTCCCATC | ||
| AD1216 | forward | CTTCATTGTGGCTGTTGCAAG | 444 |
| reverse | TCAGGGTATAGTGACCTTCC | ||
| NB2328 | forward | CCTCAGTACACAGTGAAACTC | 480 |
| reverse | GCTCTTCACAGGGATCCACA | ||
| NB3697 | forward | GGCAGAATCAGTCGAAGATC | 435 |
| reverse | GCGCCACATTTTGGAATGGG | ||
| NB5623 | forward | AGAACCAAACCCAAACAGGC | 416 |
| reverse | GTTACACGGATACAAACCCTG | ||
| TA0293 | forward | ACCATGCAGCAGAGGGAATC | 542 |
| reverse | GGATGGGTGACCATGGAGTC | ||
| TA0428 | forward | CCATCTCCTGAAACTGTAGG | 464 |
| reverse | AGGCAGTTCAAGTGGCTCTC | ||
| TA0528 | forward | CCAAAACTCCTACGGTACCG | 502 |
| reverse | TTTTTCGCAGGGGTCGGTGC | ||
| TA0721 | forward | CACTCATCTCCAGTGATACC | 452 |
| reverse | CTGGTGCTAATGTTGGAGCG | ||
| TA1050 | forward | TCATTTTCCCGATGGCAAGC | 527 |
| reverse | TTTCGACGGCACGCTTCTCC | ||
| TA1095 | forward | CCAGAGATCCTGCAGAGACA | 545 |
| reverse | GCCTTAGTGTAACACTATTCCC | ||
| Control PCR primer for template validation | |||
| NL_EF2 | forward | GAAGTTCAGTGTGTCGCCTG | 420 |
| reverse | TCTCCCAGATATCTGGCTCT | ||