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. 2008 Mar 26;3:8. doi: 10.1186/1749-8104-3-8

Figure 1.

Figure 1

RGC dendrites and putative glutamatergic postsynaptic sites visualized upon expression of td-Tomato and PSD95-YFP. (a) Whole-mount or en face view (top) and orthogonal projection (bottom) of a P7 bistratified RGC transfected with td-Tomato and PSD95-YFP. (b) Skeletonization of the cell, color-coded for depth. The ON sublamina is blue; OFF sublamina is orange. (c) Identification of PSD95-YFP puncta (cyan dots) by a semi-automated dotfinder program (see Figure 2). Td-Tomato dendritic fill is shown in red.