Table 1.

Dot-blot ELISA using Monoclonal antibodies of serogroups for N. meningitidis.

				1 week		4 weeks		1 year		13 years	4 weeks	RT
Specificity	Mabs		isotypes	dilution(a)	dilution(b)	dilution(a)	dilution(b)	dilution(a)	dilution(b)	dilution(b)	dilution(a)	dilution(b)
Group A	14-1-A	WRAIR 2D7B5B5B2	IgG1	1:25,000	1:25,000	1:25,000	1:25,000	1:25,000	1:25,000	1:5,000	1:25,000	1:25,000
Group B	2-2-B	WRAIR 5C1-3H7	IgM	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:5,000	1:25,000	1:25,000
Group C	4-2-C	WRAIR 7H9-4	IgG3	1:25,000	1:25,000	1:25,000	1:25,000	1:25,000	1:25,000	1:5,000	1:25,000	1:25,000
Group W135	7-1-W	WRAIR 6G9-7	IgM	1:10,000	1:10,000	1:10,000	1:10,000	1:10,000	1:10,000	1:10,000	1:10,000	1:10,000
Group 29EB-1-29E	WRAIR	IgM	1:5,000	1:5,000	1:5,000	1:5,000	1:5,000	1:5,000	1:2,500	1:5,000	1:5,000
Group Z	2C2-4.6	WRAIR	n/d	1:20,000	1:20,000	1:20,000	1:20,000	1:20,000	1:20,000	1:5,000	1:20,000	1:20,000
Group Y	5-2-Y	WRAIR 2C2-4.2-G6	n/d	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2000	1:2,500	1:2,500

n/d = not done.

WRAIR = Walter Reed Army Institute of Research (USA).

RT = Room Temperature (30 days).

dilution(a) (Mabs) ascites freezer -20°C.

dilution(b) (Mabs) eluted of dried filter paper.

Mabs = monoclonal antibodies