Fig. 5.

Cyp6a20 expression in olfactory sensory organs. (A and B) GFP expression in the antennae (A) and palps (B). Whole-mount antennae and palps were stained with rabbit anti-GFP (green). (C and D) GFP⁺ cells are nonneuronal. Frozen sections were stained with rabbit anti-GFP (green) and rat anti-ELAV (red). (E and F) a subset of GFP⁺ cells in the antennae (E), but not in the palps (F), coexpressed LUSH. Frozen sections were stained with chick anti-GFP (green) and rabbit anti-LUSH (red). Confirmation of colabeling of one cell (white arrow) by z-series analysis is shown below and to the right of E. Inset in E is a higher magnification view of the boxed region (arrowhead), illustrating GFP⁺ LUSH⁻ and GFP⁺ LUSH⁺ cells. In all images, TOPRO-3 (blue) was used for nuclear staining. (Scale bars, 50 μm.)