Fig. 5.

ES cell-derived neurons expressing Tlx3 exhibit an excitatory neuronal phenotype. (A) Flow cytometry profiles of GluR2-Alexa568 fluorescence in pT- or pG-expressing ES-derived cells before and after neural induction (NI). The majority of NI-pT cells express GluR2, whereas only 24.3% of NI-pG cells are positive for GluR2. (B) Large spontaneous excitatory postsynaptic currents (EPSCs) were observed in some of ES cell-derived neurons expressing the Tlx3 expression vector (Right). By contrast, EPSCs were not obvious in ES cell-derived neurons expressing the control vector (Left).